Biochemistry and Genetics: Pretest Self-Assessment and by Wilson G.N., Schroeder B., Woo J.

By Wilson G.N., Schroeder B., Woo J.

Those "500 questions, solutions, and clarification" books are designed to simulate the us clinical Licensing exam (USMLE) Step 1. every one is authored through a professional within the box and has been reviewed through scholars who've lately handed the examination. As Step 1 is turning into a growing number of scientific in nature, while appropriate, medical vignette-style questions requiring a two-step reasoning method also are integrated. the entire Pretest titles contain a "high-yield" truth part to enhance key suggestions.

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Qxd 11/14/01 10:37 AM Page 47 Gene Expression 47 70. An older man with severe emphysema is found to have decreased amounts and abnormal mobility of α1 antitrypsin (AAT) protein in his serum when analyzed by serum protein electrophoresis. Liver biopsy discloses mild scarring (cirrhosis) and demonstrates microscopic inclusions due to an engorged endoplasmic reticulum (ER). The most likely explanation for these findings is a. Defective transport from hepatic ER to the serum b. A mutation affecting the N-terminal methionine and blocking initiation of protein synthesis c.

D. e. -CCA-CCT-AGG-TTC-AGG-GCC-CCT-AGG-TTC-AGG-CCA-CCC-TAG-GTT-CAG-CCC-CTA-GGT-TCA-GG— -CCC-CCT-AGG-AGG—— 36. Which of the following results is provided by northern blot analysis? a. b. c. d. e. Detects specific base pairs Detects DNA molecules Detects RNA molecules Detects proteins Determines chromosome structure 37. The hypothetical “stimulin” gene contains two exons that encode a protein of 100 amino acids. They are separated by an intron of 100 bp beginning after the codon for amino acid 10.

Since single-nucleotide changes in the gene may not affect protein size or conformation, western blotting is generally less sensitive and specific than DNA diagnosis. 35. The answer is c. (Murray, pp 452–467. Scriver, pp 3–45. Sack, pp 1–40. ) Insertion (choice c) or deletion (choice d) of nucleotides shifts the reading frame unless the change is a multiple of 3 (choice e). Frame shifts may create unintended stop codons as in choice c. , P21A (choice b). , C63A. Deletions may be prefixed by the letter delta, as with ∆F25 (choice e).

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